What should you do if your sample is floating?


Last night I extracted the DNA fragment by using phenol-CIAA and precipitated it with 99% ethanol. The remaining ethanol caused my sample floating so that I became very panic. Fortunately, I stopped applying my sample and added more dye. The loading dye helped my sample getting down in the well…and I was very happy. Anyway, when I saw the result to my sensei that I recovered the DNA this time and solved the problem of my floating sample…my sensei said that I do not need to extract the DNA before applying to the agarose gel! Oh, no, before, he said that it was the best way, so I tried to do it very slowly but carefully…Anyway, it’s over. I recover the DNA!

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