Monthly Archives: January, 2009

What should you do if your sample is floating?


Last night I extracted the DNA fragment by using phenol-CIAA and precipitated it with 99% ethanol. The remaining ethanol caused my sample floating so that I became very panic. Fortunately, I stopped applying my sample and added more dye. The loading dye helped my sample getting down in the well…and I was very happy. Anyway, …

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PicaGene-Control Vector


Now, I am doing luciferase assay and have to make control vector. I have PGV-B (Pica Gene Vector-Basic) and pcDNA3. I have to cut CMV promoter of pcDNA3 and insert it into PGV-B poly-cloning site. Cutting a vector is not a big problem for me. The problem is when I have to extract the fragment …

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do not rely on your memory


If you do realize that your memory is not good, so I agree with my sensei: “Don’t rely on your memory” Everytime you are going to do something, please check or clarify with your references. However, try to memorize everything in a better way or you will loose all of your memory because your brain …

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Where do the DNA migrate during electrophoresis?


I have never cared about the movement of the samples when I run agarose gel electrophoresis. Now, I do realize that it was a big mistake that I ever made!!! When I was put the gel on the wrong direction, I just stop the electrophoresis for a while and then turn the gel around to …

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Analyzing a Ligation before Transformation


I am afraid if I fail again! Oh, no! It is too bad. I did it until I cut the band from the agarose gel..but I did not do DNA isolation perfectly. Last night before, I was very tired and sleepy so that I did not see the undissolved gel! That was terrible thing I …

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SDS reagents


I was going to digest my plasmids to make a new construct. When I saw the reagent to stop the restriction enzyme reaction, it was undissolved. The reagent consists of 5% SDS in 0.1 M EDTA solution. Another SDS reagent, 0.2M NaCl/1% SDS or popular as Solution II (for DNA isolation), was also undissolved. I …

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